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Recombinant PECAM-1 Antibody / CD31

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16284

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 28-330 from the human protein was used as the immunogen for the recombinant PECAM-1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

C31/6446R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant PECAM-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant PECAM-1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant PECAM-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/recombinant-pecam-1-antibody-cd31-c316446r-v9415

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant PECAM-1 antibody should be determined by the researcher.

CAS Number

9000-83-3

Location

Cell Surface and Cytoplasmic

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant PECAM-1 antibody (clone C31/6446R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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