Anti-Nitric Oxide Synthase, Neuronal Antibody

Rabbit Polyclonal Antibody specific to Neuronal NO Synthase

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 1.14.13.39, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Peptidyl-cysteine S-nitrosylase NOS1, bNOS

Gene Name

NOS1

NCBI Gene ID

UniProt

P29475

Cellular Locus

Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 19-31 of human neuronal nitric oxide synthase.

Target Antigen

Nitric oxide synthase, brain

Target

Nitric Oxide Synthase, Neuronal

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. In immunoblots, a band of approximately 155 kD is detected.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes the human variant of neuronal nitric oxide synthase (nNOS). nNOS is one of three NOS isoforms along with endothelial cell NOS (eNOS) and inducible NOS (iNOS). The three isoforms differ in primary sequence, having only 50-60% sequence identity. nNOS and iNOS can be purified from cytosol while eNOS is membrane-bound. All three NOS isoforms catalyze the formation of NO and L-citrulline from L-arginine.