Recombinant LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha

Lymphoid enhancer-binding factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q9UJU2

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 1-200) of human LEF1 protein was used as the immunogen for the recombinant LEF1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

LEF1/9403R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant LEF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant LEF1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant LEF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant LEF1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant LEF1 antibody (clone LEF1/9403R) . Strong nuclear staining of non-germinal center of cells observed. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.