Recombinant PD-L1 Antibody / B7-H1 / CD274

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

Q9NZQ7

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 190-290) of human CD274 protein was used as the immunogen for the recombinant PD-L1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PDL1/8195R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant PD-L1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant PD-L1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant PD-L1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant PD-L1 antibody should be determined by the researcher.

Location

Cytoplasm, Cell membrane

Image Legend

Western blot testing of human HDLM-2 cell lysate using recombinant PD-L1 antibody (clone rCD274/8195R) . Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated) .