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Recombinant Carbonic Anhydrase 9 Antibody / CA9

In normal kidney, CA9 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CA9 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q16790

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CA9 protein was used as the immunogen for the recombinant Carbonic Anhydrase 9 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RPN-15

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Carbonic Anhydrase 9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Carbonic Anhydrase 9 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Carbonic Anhydrase 9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Carbonic Anhydrase 9 antibody should be determined by the researcher.

Location

Cell membrane, Nucleus

Image Legend

IHC staining of FFPE human renal cell carcinoma tissue with recombinant Carbonic Anhydrase 9 antibody (clone rPN-15) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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