Recombinant Carbonic Anhydrase IX Antibody / CA9

CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CAIX is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

Q16790

Host

Rabbit

Reactivity

Human

Immunogen

The extracellular N-terminal proteoglycan-like region was used as the immunogen for the recombinant Carbonic Anhydrase IX antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CA9/9621R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Carbonic Anhydrase IX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Carbonic Anhydrase IX antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Carbonic Anhydrase IX antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Carbonic Anhydrase IX antibody should be determined by the researcher.

Location

Cell membrane, Nucleus

Image Legend

Western blot testing of human HeLa cell lysate with recombinant Carbonic Anhydrase IX antibody. Predicted molecular weight: 50-55 kDa but may be observed at higher molecular weights due to glycosylation.