Recombinant Tripartite motif-containing protein 29 Antibody / TRIM29

Tripartite motif-containing protein 29 (TRIM29) interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q14134

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 1-200) of human recombinant Tripartite motif-containing protein 29 protein was used as the immunogen for the TRIM29 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

TRIM29/9257R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Tripartite motif-containing protein 29 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Tripartite motif-containing protein 29 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Tripartite motif-containing protein 29 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Tripartite motif-containing protein 29 antibody should be determined by the researcher.

Location

Cytoplasm, Cell membrane

Image Legend

IHC staining of FFPE human esophagus tissue with recombinant Tripartite motif-containing protein 29 antibody (clone TRIM29/9257R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.