Recombinant Bcl-X Antibody

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2a or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q07817

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 1-100) of human BCL2L1 protein was used as the immunogen for the recombinant Bcl-X antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

BCL2L1/9221R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant Bcl-X antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Bcl-X antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Bcl-X antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant Bcl-X antibody should be determined by the researcher.

Location

Cytoplasm, Cell membrane, Nuclear membrane

Image Legend

IHC staining of FFPE human Hodgkins lymphoma tissue with recombinant Bcl-X antibody (clone BCL2L1/9221R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.