MDM2 Antibody

E3 ubiquitin-protein ligase Mdm2 is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53. It can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Overexpression of MDM2 protein is detected in a variety of cancers.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q00987

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human MDM2 protein fragment (within amino acids 1-300) was used as the immunogen for the MDM2 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MDM2/7943

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the MDM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MDM2 antibody is available for research use only.

Storage Conditions

Aliquot the MDM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the MDM2 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human bladder tissue with MDM2 antibody (clone MDM2/7943) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.