Opa-interacting protein 4 Antibody / OIP-4 / PRAME

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P78395

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 100-250) of human PRAME protein was used as the immunogen for the Opa-interacting protein 4 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PRAME/9189

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the PRAME antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Opa-interacting protein 4 antibody is available for research use only.

Storage Conditions

Aliquot the PRAME antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the PRAME antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm, Cell membrane

Image Legend

IHC staining of FFPE human melanoma tissue with Opa-interacting protein 4 antibody (clone PRAME/9189) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.