Recombinant Hemoglobin alpha Antibody / HBA
Hemoglobin (Hgb) is coupled to four iron-binding, methene-linked tetrapyrrole rings (heme) . The alpha and beta globin loci determine the basic Hgb structure. The globin portion of Hgb consists of two alpha chains and two beta chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between alpha and beta chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the alpha1-beta2 cleavage plane. When the two alpha1-beta2 interfaces are closely bound, Hgb has a low affinity for oxygen. Hb A, which contains two alpha chains plus two beta chains, comprises 97% of total circulating hemoglobin. The remaining 3% of total circulating hemoglobin is comprised of Hb A-2, which consists of two alpha chains plus two delta chains, and fetal hemoglobin (Hb F), which consists of two alpha chains together with two gamma chains.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P69905
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant fragment (within amino acids 1-100) of human HBA2 protein was used as the immunogen for the recombinant Hemoglobin alpha antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
HBA/9193R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Hemoglobin alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Hemoglobin alpha antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Hemoglobin alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Hemoglobin alpha antibody should be determined by the researcher.
Location
Extracellular space
Image Legend
SDS-PAGE analysis of purified, BSA-free recombinant Hemoglobin alpha antibody (clone HBA/9193R) as confirmation of integrity and purity.
Frequently Asked Questions
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