Thyroid transcription factor 1 Antibody / TTF-1 / NX2.1

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1) . TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P43699

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 1-200) of human TTF-1 protein was used as the immunogen for the Thyroid transcription factor 1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

NX2.1/9036

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Thyroid transcription factor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Thyroid transcription factor 1 antibody is available for research use only.

Storage Conditions

Aliquot the Thyroid transcription factor 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Thyroid transcription factor 1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human thyroid tissue with Thyroid transcription factor 1 antibody (clone NX2.1/9036) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.