Recombinant NCAM-L1 Antibody / CD171 / L1CAM

L1 (L1 cell adhesion molecule) also known as CAML1, CD171, and HSAS is an adhesion molecule which plays an important role in the nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus) . This mouse monoclonal antibody recognizes the sequence immediately NH2-terminal to the tyrosine-based motif and binds L1 only when Y1176 is dephosphorylated. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P32004

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human L1CAM protein was used as the immunogen for the recombinant NCAM-L1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

L1CAM/9146R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant NCAM-L1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant NCAM-L1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant NCAM-L1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant NCAM-L1 antibody should be determined by the researcher.

Location

Cytoplasm, Cell membrane

Image Legend

IHC staining of FFPE human brain tissue with recombinant NCAM-L1 antibody (clone L1CAM/9146R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.