Recombinant Carbamoyl phosphate synthetase 1 Antibody / CPS1
This MAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1) . This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P31327
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant full-length human CPS1 protein was used as the immunogen for the recombinant Carbamoyl phosphate synthetase 1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
CPS1/9151R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Carbamoyl phosphate synthetase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Carbamoyl phosphate synthetase 1 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Carbamoyl phosphate synthetase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Carbamoyl phosphate synthetase 1 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human liver tissue lysate with recombinant Carbamoyl phosphate synthetase 1 antibody. Predicted molecular weight ~165 kDa.
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