Recombinant S100P Antibody / MIG9

S100P is a 95-amino-acid protein and a member of the S100 family. S100P has been shown to mediate tumor growth, metastasis and invasion through the binding of Ca2+ ions, receptor for advanced glycation end products, cytoskeletal protein ezrin, calcyclin-binding protein/Siah-1-interacting protein and cathepsin D. S100P highly expressed in human placenta, gastrointestinal tract, and esophageal mucosa, but always negative in pancreas and liver. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P could potentially serve as diagnostic marker, prognostic/predictive indicator and therapy target for different carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P25815

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment corresponding to the C-terminus of human S100P/MIG9 protein was used as the immunogen for the recombinant S100P antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RS100P/9254

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant S100P antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant S100P antibody is available for research use only.

Storage Conditions

Aliquot the recombinant S100P antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant S100P antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with recombinant S100P antibody (clone rS100P/9254) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.