Recombinant PAX7 Antibody
The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma) .
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P23759
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment (amino acids 301-505) of human PAX7 protein was used as the immunogen for the recombinant PAX7 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2b κ
Clone
RPAX7/9325
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant PAX7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant PAX7 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant PAX7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/recombinant-pax7-antibody-rpax79325-v5518
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant PAX7 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
SDS-PAGE analysis of purified, BSA-free recombinant PAX7 antibody (clone rPAX7/9325) as confirmation of integrity and purity.
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