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S100A1 Antibody

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B) . This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Product Specifications

Specifications

Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P23297

Host

Rabbit

Reactivity

Human

Immunogen

Purified human S-100 protein was used as the immunogen for the S100A1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

S100A1/6374R

Type

Recombinant

Applications

IF, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the S100A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This S100A1 antibody is available for research use only.

Storage Conditions

Aliquot the S100A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/s100a1-antibody-s100a16374r-v5516

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the S100A1 antibody should be determined by the researcher.

CAS Number

9000-83-3

Location

Cytoplasm

Image Legend

SDS-PAGE analysis of purified, BSA-free S100A1 antibody (clone S100A1/6374R) as confirmation of integrity and purity.

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