S100A1 Antibody
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B) . This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
Product Specifications
Specifications
Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P23297
Host
Rabbit
Reactivity
Human
Immunogen
Purified human S-100 protein was used as the immunogen for the S100A1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
S100A1/6374R
Type
Recombinant
Applications
IF, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the S100A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This S100A1 antibody is available for research use only.
Storage Conditions
Aliquot the S100A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/s100a1-antibody-s100a16374r-v5516
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the S100A1 antibody should be determined by the researcher.
CAS Number
9000-83-3
Location
Cytoplasm
Image Legend
SDS-PAGE analysis of purified, BSA-free S100A1 antibody (clone S100A1/6374R) as confirmation of integrity and purity.
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