Recombinant CD44v6 Antibody

This antibody recognizes an epitope encoded by exon v6 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16070

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment corresponding to the v3-v10 domain of human CD44 was used as the immunogen for the recombinant CD44v6 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CD44V6/9400R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant CD44v6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant CD44v6 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CD44v6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant CD44v6 antibody should be determined by the researcher.

Location

Cell membrane

Image Legend

IHC staining of FFPE human esophagus tissue with recombinant CD44v6 antibody (clone CD44V6/9400R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.