VEGF Antibody / Vascular Endothelial Growth Factor
This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-3 µg/mL
UniProt
P15692
Host
Mouse
Reactivity
Human
Immunogen
Human VEGF189 recombinant protein was used as the immunogen for the VEGF antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VG76e
Applications
IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the VEGF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This VEGF antibody is available for research use only.
Storage Conditions
Aliquot the VEGF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the VEGF antibody should be determined by the researcher.
Location
Secreted
Image Legend
SDS-PAGE analysis of purified, BSA-free VEGF antibody (clone VG76e) as confirmation of integrity and purity.
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