Fatty Acid Binding Protein 4 Antibody / FABP4

Fatty acid-binding proteins, designated FABPs, are a family of homologous, cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP) . The human A-FABP gene is organized into 4 exons, maps to chromosome 8q21.13, and encodes a 132 amino acid protein. A-FABP protein comprises approximately 1% of the total cytosolic protein in human adipose tissue.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15090

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 1-132) of human FABP4 protein was used as the immunogen for the Fatty Acid Binding Protein 4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

FABP4/9086R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Fatty Acid Binding Protein 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Fatty Acid Binding Protein 4 antibody is available for research use only.

Storage Conditions

Aliquot the Fatty Acid Binding Protein 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Fatty Acid Binding Protein 4 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human placental tissue with Fatty Acid Binding Protein 4 antibody (clone FABP4/9086R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.