KRT5/6 Antibody / Cytokeratin 5/6

Cytokeratin 5 is a basic protein that is closely related to cytokeratin 6. They share similar tissue distribution and are found in various proportions in many non-keratinizing stratified squamous epithelia eg. tongue mucosa, as well as in basal epithelia of trachea, basal cells of epidermis, hair follicles, sebaceous and sweat glands of skin, luminal cells of the mammary gland, basal cells of prostate, urothelium, vagina and endocervical mucosa. Cytokeratins 5 and 6 are also expressed in basal cell epitheliomas, squamous cell carcinomas of skin, tongue, epiglottis and of the rectal-anal region. Cytokeratin 5&6 Ab is a useful aid for classification of epithelioid mesotheliomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P13647, P02538

Host

Mouse

Reactivity

Human

Immunogen

A recombinant protein fragment common to human Cytokeratin 5 and Cytokeratin 6 proteins was used as the immunogen for the KRT5/6 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KRT5.6/9103

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the KRT5/6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KRT5/6 antibody is available for research use only.

Storage Conditions

Aliquot the KRT5/6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the KRT5/6 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human prostate carcinoma tissue with KRT5/6 antibody (clone KRT5.6/9104) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.