Myosin 7 Antibody / MYH7

Myosin heavy chains are ubiquitous Actin-based motor proteins that convert the chemical energy derived from ATP hydrolysis into the mechanical energy that drives diverse motile processes in eukaryotic cells, including cytokinesis, vesicular transport and cellular locomotion. Muscle myosin is a heterohexamer consisting of two myosin heavy chains and two associated nonidentical pairs of myosin light chains. The seven myosin heavy chain isoforms that predominate in mammalian skeletal muscles include two developmental isoforms, MHC-embryonic (MYH3) and MHC-perinatal (MYH8) ; three adult skeletal muscle isoforms, MHC IIa (MYH2), MHC IIb (MYH4) and MHC IIx/d (MYH1) ; and MHC-?/slow (MYH7 or MHC-?), which is also expressed in cardiac muscle. Research indicates that mutations of the MYH7 gene causes hypertrophic cardiomyopathy.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P12883

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 1150-1350) of human Myosin 7 protein was used as the immunogen for the Myosin 7 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MYH7/9184

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Myosin 7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Myosin 7 antibody is available for research use only.

Storage Conditions

Aliquot the Myosin 7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Myosin 7 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human skeletal muscle tissue with Myosin 7 antibody (clone MYH7/9184) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.