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CK-BB Antibody / Creatine Phosphokinase-B / CKB

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P12277

Host

Mouse

Reactivity

Human, Mouse, Rat, Hamster, Guinea pig

Immunogen

Recombinant human full-length CKB protein was used as the immunogen for the CKB antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CKBB/6871

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CK-BB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CK-BB antibody is available for research use only.

Storage Conditions

Aliquot the CK-BB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CK-BB antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain and Guinea pig Brain tissue lysates using CK-BB antibody (clone CKBB/6871) . Predicted molecular weight ~43 kDa.

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