Gamma Enolase Antibody / ENO2 / NSE gamma
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found predominantly in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin, ?Chromogranin A, and Neurofilament.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P09104
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment of human NSE gamma (within amino acids 150-300) was used as the immunogen for the Gamma Enolase antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ENO2/9046
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Gamma Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Gamma Enolase antibody is available for research use only.
Storage Conditions
Aliquot the Gamma Enolase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Gamma Enolase antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human 1) HepG2 and 2) A549 cell lysate with Gamma Enolase antibody (clone ENO2/9046) . Predicted molecular weight ~47 kDa.
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