Recombinant CK8/18 Antibody / Cytokeratin 8/18
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P05787, P05783
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant full-length human CK8 and CK18 proteins were used as the immunogen for the recombinant CK8/18 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
KRT8.18/6579R
Type
Recombinant
Applications
FACS, IF, IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant CK8/18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant CK8/18 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CK8/18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CK8/18 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
Documents
Frequently Asked Questions
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