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Recombinant CD54 Antibody / ICAM-1

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1), also called CD54. It has 7 potential N-linked glycosylation sites. ICAM-1/CD54 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P05362

Host

Rabbit

Reactivity

Human

Immunogen

IL-1B activated human vascular endothelial cells expressing ICAM-1/CD54 protein were used as the immunogen for the recombinant CD54 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ICAM1/9379R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant CD54 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant CD54 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CD54 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CD54 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

SDS-PAGE analysis of purified, BSA-free recombinant CD54 antibody (clone ICAM1/9379R) as confirmation of integrity and purity.

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