Recombinant ALPP Antibody / Placental Alkaline Phosphatase

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P05187

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human Placental Alkaline Phosphatase protein was used as the immunogen for the recombinant ALPP antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ALPP/9109R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant ALPP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant ALPP antibody is available for research use only.

Storage Conditions

Aliquot the recombinant ALPP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant ALPP antibody should be determined by the researcher.

Location

Cytoplasm, Cell membrane

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant ALPP antibody (clone ALPP/9109R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) .