Recombinant Thymidylate Synthase Antibody / TYMS

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45) . It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P04818

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human thymidylate synthase protein fragment (within amino acids 1-200) was used as the immunogen for the recombinant Thymidylate Synthase antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

TYMS/9091R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Thymidylate Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Thymidylate Synthase antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Thymidylate Synthase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Thymidylate Synthase antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human prostate carcinoma tissue with recombinant Thymidylate Synthase antibody (clone TYMS/9091R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.