P53 Tumor Suppressor Protein Antibody
P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P04637
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human full-length TP53 protein was used as the immunogen for the p53 Tumor Suppressor Protein antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
TP53/7002R
Type
Recombinant
Applications
FACS, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the p53 Tumor Suppressor Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This p53 Tumor Suppressor Protein antibody is available for research use only.
Storage Conditions
Aliquot the p53 Tumor Suppressor Protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the p53 Tumor Suppressor Protein antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
SDS-PAGE analysis of purified, BSA-free p53 antibody (clone TP53/7002R) as confirmation of integrity and purity.
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