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Recombinant ErbB2 Antibody

C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P04626

Host

Mouse

Reactivity

Human

Immunogen

Recombinant protein corresponding to the cytoplasmic domain (within amino acids 955-1255) of human c-erbB2/HER-2 was used as the immunogen for the recombinant ErbB2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RERBB2/9538

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant ErbB2 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant ErbB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/recombinant-erbb2-antibody-rerbb29538-v5422

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant ErbB2 antibody should be determined by the researcher.

CAS Number

9000-83-3

Location

Cell Surface

Image Legend

IHC staining of FFPE human breast cancer tissue with recombinant ErbB2 antibody (clone rERBB2/9538) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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