Recombinant MBP Antibody / Myelin Basic Protein
Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. Clone rMBP/9301 is used in a two-site ELISA with clone MBP/4273 (cat # V8677)<a href=../tds/myelin-basic-protein-antibody-mbp-4273-v8677></a>. Both are used interchangeably as capture and detection antibody.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P02686
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment (within amino acids 1-157) of human Myelin Basic Protein was used as the immunogen for the recombinant MBP antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2b κ
Clone
RMBP/9301
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant MBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant MBP antibody is available for research use only.
Storage Conditions
Aliquot the recombinant MBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/recombinant-mbp-antibody-myelin-basic-protein-rmbp9301-v5403
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant MBP antibody should be determined by the researcher.
Image Legend
Western blot testing of human brain tissue with recombinant MBP antibody. Expected molecular weight: 22-37 kDa.
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