Recombinant Cystatin A Antibody / CSTA

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01040

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human CSTA protein (within amino acids 1-98) was used as the immunogen for the recombinant Cystatin A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CSTA/9087R

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Cystatin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Cystatin A antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Cystatin A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Cystatin A antibody should be determined by the researcher.

Location

Cell Surface, Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant Cystatin A antibody (clone CSTA/9087R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.