EGFR Antibody / EGF Receptor
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-3 µg/mL, Western blot: 2-4 µg/mL
UniProt
P00533
Host
Mouse
Reactivity
Human
Immunogen
Purified EGF Receptor protein from A431 cells was used as the immunogen for the EGFR antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
225
Applications
ELISA, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the EGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EGFR antibody is available for research use only.
Storage Conditions
Aliquot the EGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the EGFR antibody should be determined by the researcher.
Location
Cell Surface
Image Legend
SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone 225) as confirmation of integrity and purity.
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