Recombinant Uroplakin 1B Antibody / UPK1B

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.UPK1B is expressed by terminally differentiated urothelial cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O75841

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 109-229) of human Uroplakin 1B/UPK1B protein was used as the immunogen for the recombinant Uroplakin 1B antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

UPK1B/9258R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Uroplakin 1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Uroplakin 1B antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Uroplakin 1B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Uroplakin 1B antibody should be determined by the researcher.

Location

Membrane

Image Legend

IHC staining of FFPE human bladder carcinoma tissue with recombinant Uroplakin 1B antibody (clone UPK1B/9258R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.