Mouse IgG1 Antibody / Isotype Control for Mouse

To serve as a true negative control for various procedures, this mouse monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: application dependent , Immunofluorescence: application dependent, Immunohistochemistry (FFPE) : application dependent, Western blot: application dependent

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human IG1R protein was used as the immunogen for the mouse IgG1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ICG1/1331

Applications

FACS, IF, IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the mouse IgG1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This mouse IgG1 antibody is available for research use only.

Storage Conditions

Aliquot the mouse IgG1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the mouse IgG1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human heart tissue with mouse IgG1 antibody (clone ICG1/ 1331) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. No staining was observed.