Recombinant HER-2 Antibody / ErbB2

C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P04626

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 1155-1255) of human HER-2/ERBB2 protein was used as the immunogen for the recombinant HER-2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RERBB2/9401

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant HER-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant HER-2 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant HER-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant HER-2 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human breast carcinoma tissue with recombinant HER-2 antibody (clone rERBB2/9401) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.