Lysophospholipase 1 Antibody / LYPLA1 / LPL-I

Acyl-protein thioesterase 1 is an enzyme that in humans is encoded by the LYPLA1 gene. This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

O75608

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids H35-D230) was used as the immunogen for the Lysophospholipase 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Lysophospholipase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Lysophospholipase 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Lysophospholipase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Lysophospholipase 1 antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane, nuclear membrane

Image Legend

IHC staining of FFPE human rectum adenocarcinoma tissue with Lysophospholipase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.