MAGED4 Antibody / Melanoma-associated antigen D4

MAGE family member D4 is a protein that in humans is encoded by the MAGED4 gene. MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique. Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription. This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q96JG8

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (F51-R741) was used as the immunogen for the MAGED4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the MAGED4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MAGED4 antibody is available for research use only.

Storage Conditions

After reconstitution, the MAGED4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MAGED4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human bladder urothelial carcinoma tissue with MAGED4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.