MARCH2 Antibody / MARCHF2 / Membrane-associated RING finger protein 2

MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q9P0N8

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (M1-V246) was used as the immunogen for the MARCH2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the MARCH2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MARCH2 antibody is available for research use only.

Storage Conditions

After reconstitution, the MARCH2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MARCH2 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with MARCH2 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.