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NUDCD3 Antibody / NudC domain-containing protein 3

NudC domain-containing protein 3 is a protein that in humans is encoded by the NUDCD3 gene. The product of this gene functions to maintain the stability of dynein intermediate chain. Depletion of this gene product results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. The protein localizes to the Golgi apparatus during interphase, and levels of the protein increase after the G1/S transition.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8IVD9

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (M1-Y271) was used as the immunogen for the NUDCD3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NUDCD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NUDCD3 antibody is available for research use only.

Storage Conditions

After reconstitution, the NUDCD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NUDCD3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with NUDCD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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