NEEP21 Antibody / Neuron-enriched endosomal protein of 21 kDa / NSG1

NSG1, also known as Neuron-specific protein family member 1 and Neuron-enriched endosomal protein of 21 kDa (NEEP21), contains an N-terminal nuclear localization motif and a central hydrophobic segment. It localizes to the nucleus in mitotic neuronal cell cultures and to the cytoplasm in postmitotic neurons. Mouse and human NEEP21/NSG1 share 97% sequence identity and they differs from each other by only 5 amino acid residues. The protein is expressed in all regions of rat brain. In hamster, it was preferentially expressed in adult brain.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P42857

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (M1-Q176) was used as the immunogen for the NEEP21 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NEEP21 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NEEP21 antibody is available for research use only.

Storage Conditions

After reconstitution, the NEEP21 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NEEP21 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human large B-cell lymphoma tissue with NEEP21 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.