L3MBTL3 Antibody / Lethal (3) malignant brain tumor-like protein 3

This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q96JM7

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (Q11-R686) was used as the immunogen for the L3MBTL3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the L3MBTL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This L3MBTL3 antibody is available for research use only.

Storage Conditions

After reconstitution, the L3MBTL3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the L3MBTL3 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human U-251 cells with L3MBTL3 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= L3MBTL3 antibody.