MYDGF Antibody / Myeloid-derived growth factor

Human MYDGF, also called Myeloid-derived growth factor, Stromal cell-derived growth factor SF20 and C19orf10, and formerly known as IL25, is a bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. MYDGF stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q969H8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (V32-L173) was used as the immunogen for the MYDGF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the MYDGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MYDGF antibody is available for research use only.

Storage Conditions

After reconstitution, the MYDGF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MYDGF antibody should be determined by the researcher.

Location

Cytoplasm, secreted

Image Legend

Western blot testing of 1) rat liver, 2) rat NRK, 3) mouse ovary, 4) mouse stomach and 5) mouse liver tissue lysate with MYDGF antibody. Predicted molecular weight ~19 kDa, commonly observed at 14-19 kDa.