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Phospho-Histone H3.3 Antibody (pS31)

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000

UniProt

P84243

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide derived from the area surrounding phosphorylated serine 31 of human Histone H3.3 was used as the immunogen for the Phospho-Histone H3.3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

28H13

Type

Recombinant

Applications

WB

Purity

Affinity chromatography

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the Phospho-Histone H3.3 antibody at -20oC.

Limitations

This Phospho-Histone H3.3 antibody is available for research use only.

Storage Conditions

Store the Phospho-Histone H3.3 antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-Histone H3.3 antibody should be determined by the researcher.

Image Legend

Western blot testing of lysate from HeLa cells treated with Calyculin A.

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