NUCKS1 Antibody / Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1

Nuclear ubiquitous casein and cyclin-dependent kinases substrate is a protein that in humans is encoded by the NUCKS1 gene. This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemsitry (FFPE) : 2-5 µg/mL

UniProt

Q9H1E3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids MSRPVRNRKVVDYSQFQE were used as the immunogen for the NUCKS1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF, IHC-P

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NUCKS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NUCKS1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NUCKS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NUCKS1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human rectum adenocarcinoma tissue with NUCKS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.