Nucleolar protein 2 Antibody / NOP2 / NSUN1

Enables RNA binding activity. Involved in positive regulation of cell population proliferation; regulation of signal transduction by p53 class mediator; and ribosomal large subunit assembly. Located in nucleolus.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P46087

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (L210-Q747) was used as the immunogen for the Nucleolar protein 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Nucleolar protein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Nucleolar protein 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Nucleolar protein 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Nucleolar protein 2 antibody should be determined by the researcher.

Location

Nucleolus

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolar protein 2 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.