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NTH1 Antibody / Endonuclease III-like protein 1 / NTHL1

Endonuclease III-like protein 1 is an enzyme that in humans is encoded by the NTHL1 gene. The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P78549

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (H52-L312) was used as the immunogen for the NTH1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NTH1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NTH1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NTH1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NTH1 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A549 cells with NTH1 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.

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