NR5A2 Antibody / LRH1

NR5A2 (nuclear receptor subfamily 5, group A, member 2) also known as liver receptor homolog-1 (LRH-1) is a protein that in humans is encoded by the NR5A2 gene. LRH-1 is a member of the nuclear receptor family of intracellular transcription factors. LRH-1 plays a critical role in the regulation of development, cholesterol transport, bile acid homeostasis and steroidogenesis. LRH-1 is important for maintaining pluripotence of stem cells during embryonic development. Liver receptor homolog-1 has been shown to interact with the small heterodimer partner.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O00482

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (K192-A508) was used as the immunogen for the NR5A2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NR5A2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NR5A2 antibody is available for research use only.

Storage Conditions

After reconstitution, the NR5A2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NR5A2 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human colorectal adenocarcinoma tissue with NR5A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.