C7orf47 Antibody / PPP1R35

PPP1R35 (Protein phosphatase 1 regulatory subunit 35), also called C7orf47, is a putative regulator of protein phosphatase-1. It is a centrosomal protein that regulates centriole elongation and centriole-to-centrosome conversion. Predicted to enable phosphatase binding activity and protein phosphatase inhibitor activity. Predicted to be involved in negative regulation of phosphatase activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8TAP8

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids E27-A253) was used as the immunogen for the C7orf47 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the C7orf47 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This C7orf47 antibody is available for research use only.

Storage Conditions

After reconstitution, the C7orf47 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the C7orf47 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with C7orf47 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.