NANP Antibody / N-acylneuraminate-9-phosphatase

NANP (N-acylneuraminate-9-phosphatase), also known as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4), is a 248 amino acid protein that belongs to the haloacid dehalogenase (HAD) family and is responsible for dephosphorylating Neu5Ac-9-phosphate to form N-acetylneuraminate. Characteristic of the HAD phosphatase family, the catalytic activity of NANP is dependent upon the presence of magnesium and is inhibited by vanadate and calcium.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8TBE9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids R5-M246) was used as the immunogen for the NANP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NANP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NANP antibody is available for research use only.

Storage Conditions

After reconstitution, the NANP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NANP antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human thyroid papillary carcinoma tissue with NANP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.